N2a细胞增殖的痒病小鼠适应毒株RML的致病特性分析

doi:10.11843/j.issn.0366-6964.2015.02.012

Abstract

Abstract:

To identify the pathogenic characteristics of mouse-adapted Scrapie Strain RML propagated in N2a cell line，we intracerebrally inoculated mice with the lysate of Sc-N2a cell culture.After 200±28 days’ incubation period，all inoculated C57BL/6J mice appeared typical ataxia symptoms of TSEs.The PrP^Sc from both brain tissues and Sc-N2a cells shared the same biochemical aspects.Western blotting result confirmed that they had the same glycosylation patterns，the same size distribution of PK digestion fragments，with higher levels of monoglycosylated.The pathological changes of brain sections from inoculated mice at the terminal stage were analyzed by HE stain and IHC assay.Vacuoles were mainly found in brain stem，cortex，cerebellum and thalamus，while PrP^Sc deposits mainly in cortex，hippocampus，thalamus，cerebellum.The results of mouse regression test showed that the PrP^Scpropagated in N2a cell line had pathogenicity，and kept the the stain characteristics of mouse-adapted scrapie strain RML.The Sc-N2a cell line is an ideal model for the study of TSEs.

CLC Number:

S852.659.7

WU Xiao-dong，ZOU Yan-li，LIU Yu-tian，LI Jin-ming，ZHANG Yong-qiang，LIU Shan，WANG Zhi-liang. Pathogenic Characteristics of Mouse-adapted Scrapie Strain RML Propagated in N2a Cell Line[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 257-263.

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Pathogenic Characteristics of Mouse-adapted Scrapie Strain RML Propagated in N2a Cell Line

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